The Intracellular Distribution of Ca²⁺ in Schizosaccharomyces pombe Shown by Cameleon Calcium Indicator YC2.1

Abstract: We transformed the recombined yellow cameleon 2.1 (YC2.1) gene into the Schizosaccharomyces pombe and observed the distribution of free calcium concentration in the cytoplasm shown by expressed YC2.1. The results showed that the calcium fluorescence ratio of YC2.1 appeared mainly around the peripheral area of the cytoplasm in the fission yeast cell， less in the center area of the cytoplasm in which occupied by the organelles and nuclear. In contrast， the calcium fluorescence of fluo-3 was higher in the center of the cytoplasm not than around the peripheral area of the cytoplasm because fluo-3 was sequestered into organelles in the cell. Therefore， fluo-3 could not reflect the true distribution of calcium in the cytoplasm. Our data provided that the recombinant yellow cameleon calcium indicator is better than the traditional chemical fluorescence indicator fluo-3 for measuring of free calcium in cytoplasm， and will promote studying of distribution and role of intracellular calcium in yeast cell proliferation.

CSTR: 32200.14.cjcb.2005.05.0017