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The Effects of IL-18 Gene Transfection on the Proliferation of C6 Glioma Cell
Chang-Wen Jiang, Yun-Li Yan*, Wei -Dong Ma, Li-Fen Zheng, Li-Dong Zhai, Wen-Ling Li, Cui-Li Cao
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Abstract: To explore the effects of IL-18 gene transfection on the proliferation of C6 glioma cell. The proliferation and cell cycle distribution of C6/IL-18 and C6 cells were detected with flow cytometry and MTT methods. The expression of PCNA and vimentin were determined with immunocytochemical assay. It was shown that the proliferation of C6/IL-18 cells was reduced compared with its parent C6 cells. The G0/G1 cells were more and G2/M cells became less about C6/IL-18 cells than those of the C6 cells. The expression of the PCNA and vimentin were lower in C6/IL-18 cells than those in C6 cells. It was suggested that IL-18 gene transfection could inhibit the proliferation and decrease malignant degree of C6 cells.
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