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Isolation and Culture of Endothelial Progenitor Cells from Pig Peripheral Bl
Shu-Yan Chen*, Fei Wang, Qin Zhou, Xue-Yun Yan, Qin Xu, Hong Shu,Ye-Zhi Rong
Department of Cardiology, Xinhua Hospital Affiliated to Shanghai Second Medical University, Shanghai 200092, China
Abstract: Mononuclear cells were isolated from pig peripheral blood by density-gradient centrifugation and cultured in EGM-2. After 10 to 14 days of culture， adherent cells forming colony were selected. Immunohistochemistry and fluorescent staining was performed to identify the expressing of EC-specific antigens and functions. Results show that after 10 days of culture， the adherent cells resulted in a spindle-shaped， EC-like morphology. These cells have the ability to endocytose ac-LDL and bind BS-1 lectin. They also could be shown to have EC specific markers such as CD31， flk-1 and vWF. These results demonstrate that endothelial progenitor cells (EPCs) can be isolated and cultured from pig peripheral blood and provide a cell source for the further investigation of autologous EPCs transplantation contributing to promote angiogenesis in pig chronic cardiac ischemia.
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