The Effect of LncRNA DLEU2 on the Malignant Biological Behavior of Ovarian Cancer Cells by Regulating the miR-30a-5p/SOX4 Signaling Pathway
QI Lining1, WANG Qi1, ZHU Jihong1, GENG Yanhong1, GE Xinmiao2, WEI Xujing3*, LIU Caihui1
This study aimed to investigate the effect of LncRNA DLEU2 on the malignant biological behavior of ovarian cancer cells by regulating the miR-30a-5p/SOX4 signaling pathway. Phenotypic screening of ovarian cancer cells (SK-OV-3, OVCAR-8, A2780, OV90), ovarian cancer cells SK-OV-3 were selected and assigned into control group, si-NC group, si-DLEU2 group, mimic NC group, miR-30a-5p mimic group, si-DLEU2+inhibitor NC group, and si-DLEU2+miR-30a-5p inhibitor group. qRT-PCR was applied to detect the mRNA expression levels of LncRNA DLEU2, miR-30a-5p, and SOX4. CCK-8 method was applied to detect cell proliferation. Scratch experiment was applied to detect cell migration. Transwell method was applied to detect cell invasion. Hoechst 33258 staining was applied to detect cell apoptosis. Western blot was applied to detect the expression level of SOX4 protein. The dual luciferase reporter gene assay was applied to detect the interaction between LncRNA DLEU2 and miR-30a-5p, and between miR-30a-5p and SOX4. The results showed that compared with normal human ovarian cells, the expression of LncRNA DLEU2 and SOX4 mRNA in SK-OV-3 increased, while the expression of miR-30a-5p decreased (P<0.05). Compared with the control group and si-NC group, the expression of LncRNA DLEU2, expression of SOX4, the abilities of cell proliferation, migration, and invasion were lower in SK-OV-3 cells in the si-DLEU2 group, while the expression of miR-30a-5p and apoptosis rate were higher (P<0.05). The effect of transfecting miR-30a-5p mimic on SK-OV-3 cells was the same as transfecting si-DLEU2. Compared with the si-DLEU2+inhibitor NC group, the abilities of proliferation, migration, and invasion of SK-OV-3 cells in si-DLEU2+miR-30a-5p inhibitor group were higher, the expression of SOX4 was higher, and miR-30a-5p expression and apoptosis rate were lower (P<0.05). The dual luciferase reporter gene experiment showed that LncRNA DLEU2 had a targeted relationship with miR-30a-5p, and miR-30a-5p had a targeted relationship with SOX4. In conclusion, interference with LncRNA DLEU2 may upregulate miR-30a-5p expression, inhibit SOX4 expression, and suppress the malignant biological behavior of ovarian cancer cells.
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