The Culture of Human Bone Marrow Mesenchymal Stem Cells

Abstract: Bone marrow mesenchymal stem cells (BMMSCs) are a kind of adult stem cells with multiple potential functions in cell proliferation and differentiation and will be wildly used for cell therapy and tissue engineering in the future. Many factors are reviewed in this paper which include donors' age， isolation methods， cell culture density， surface materials and their effects on cell proliferation. It is emphasized that the development of different substitution， such as growth factors and human autologous serum， for fetal bovine serum (FBS) to avoid the risk from some viruses. The BMMSCs introduced by the plasmid with telomerase gene have shown stronger proliferation ability and multiple differentiation potential. The development of perfusion culture systems will make the preparation of BMMSCs more suitable for clinical application

CSTR: 32200.14.cjcb.2006.06.0004