Screening of Antiviral Drugs to Control the Replication of Oncolytic Adenovirus YSCH-01

LIU Min^1#, QIAN Wei^1#, ZHANG Tingting², FANG Xianlong², ZHANG Kangjian^2,3, TAO Junjia^1
*

( ¹Shanghai Fengxian District Central Hospital, Shanghai 201400, China;² Shanghai Yuansong Biotechnology Co., LTD, Shanghai 201400, China;³ Institute of Intelligent Biomaterials, School of Materials Science and Engineering, Zhejiang University of Science and Technology, Hangzhou 310018, China)

Abstract:

The purpose of this article is to select the drugs which can effectively inhibit the recombinant oncolytic adenovirus YSCH-01 replication through in vitro experiments, to provide guidance for ensuring safety use of adenovirus products in clinical. First, five drugs commonly reported in the literature with potential to inhibit adenovirus were selected. The toxicity of these five drugs on human cells was tested by CCK-8 (cell counting kit-8) and Cedutinib, the most cytotoxic drug, was excluded. Then, crystal violet staining was used to detect the inhibitory effect of the remaining four drugs on YSCH-01 killing ability. At the same time, the inhibitory effect of these 4 drugs on YSCH-01 replication was measured. The results showed that Cidofovir and Stavudine significantly inhibited the replication and killing ability of YSCH-01, followed by Ganciclovir. Further, the half inhibitory concentrations of Cidofovir and Stavudine against YSCH-01 were determined and the SI values of the two drugs were calculated. This study showed that Cidofovir and Stavudine had little adverse effect on human cells but shown strong inhibition against adenovirus YSCH-01, which could be used as standby safe drugs for clinical application of adenovirus YSCH-01.

CSTR: 32200.14.cjcb.2023.04.0007