Effect of Inhibiting of circPVT1 on the Proliferation and Apoptosis of Keloid Fibroblasts and Its Related Mechanisms

In order to investigate the effect of circPVT1 on the proliferation and apoptosis of keloid fibroblasts and its molecular mechanism, keloid fibroblasts were divided into si-NC group, si-circPVT1 group, miR-NC group, miR-194-5p group, si-circPVT1+anti-miR-NC group and si-circPVT1+anti-miR-194- 5p group. The expression of circPVT1 and miR-194-5p was tested by RT-qPCR; the cell viability was tested by the tetramethylazolium salt colorimetric method (MTT); the cell apoptosis was tested by flow cytometry; Western blot was used to detect protein expression; dual luciferase reporter experiment was used to detect the targeting relationship between circPVT1 and miR194-5p. The results showed that the expression level of circPVT1 in keloid fibroblasts increased, and the expression level of miR-194-5p decreased (P<0.05). After knockdown of circPVT1 expression or overexpression of miR-194- 5p, the activity of keloid fibroblasts, the expression of CyclinD1, Bcl-2, Caspase-3 and PARP all showed a significant downward trend, while the apoptosis rate, the expression of Bax and p21 indicators showed a significant upward trend (P<0.05). The strength of miR-194-5p expression was related to circPVT1, and the latter could target and regulate the former; knockdown circPVT1 expression could inhibit the proliferation of keloid fibroblasts and promote cell apoptosis by up-regulating miR-194-5p. This leads to the conclusion: down-regulation of circPVT1 expression can stimulate the expression of miR-194-5p, thereby inhibiting the proliferation of keloid fibroblasts and promoting cell apoptosis.

CSTR: 32200.14.cjcb.2021.12.0004