The Effects of Rictor on Decidualization of Mouse Stromal Cells and Its Expression in Human Spontaneous Abortion Decidual Tissues
HE Limin1,2, LI Jian1, ZHANG Yue1, LIU Xueqing1*
This study was to investigate the effects of Rictor and SGK1 on decidualization of mouse stromal cells and their expression in human spontaneous abortion decidual tissues. Mouse stromal cells were isolated and cultured. siRNA silenced Rictor gene of mouse stromal cells and induced decidualization artificially. The protein and mRNA expressions of Rictor and SGK1 were detected by Western blot and Real-time PCR. The mRNA levels of Dtprp, a marker of decidualization, was detected by Real-time PCR. In addition, SGK1 was overexpressed to detect Dtprp mRNA levels. The decidual tissue samples of sterile fresh spontaneous abortion and normal induced abortion at 8 to 10 weeks of gestation were selected as the experimental study group (spontaneous abortion group, n=17) and normal control group (normal pregnancy group, n=34). The protein and mRNA expression levels of Rictor and SGK1 were detected by Western blot, immunohistochemistry and Real-time PCR. The results showed that the protein and mRNA expression levels of Rictor and SGK1 were significantly decreased after silencing the Rictor gene, and the decidualization index Dtprp mRNA was also significantly decreased. However, after the overexpression of SGK1, the decidualization index Dtprp mRNA was increased compared with before, and the difference was statistically significant. Compared with the normal pregnancy group, the protein and mRNA expression levels of Rictor and SGK1 were significantly decreased in the spontaneous abortion group. In this study, Rictor inhibited stromal cell decidualization by affecting SGK1 expression. The expression of Rictor and SGK1 in the spontaneous abortion group was significantly lower than that in the normal group, which might be one of the causes of abortion
CN
EN