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Hui Li*, Qiao Li, Zhen-Yu Zuo, Zan Zhou, Chun Cui1, Lei Xiao1*
Reproductive Center, Xiangtan Central Hospital, Xiangtan 411100, China; 1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Abstract: After the establishment and development of induced pluripotent stem cell (iPS cell) technique， the iPS cells from various species have been reported. To monitor the mutation of chromosomes in iPS cells， the karyotype analysis became more and more important. We established the protocol to do karyotyping for human， pig and rat iPS cells. We also investigated the factors involved in the quality of the karyotyping including colchicine concentration， duration of colchicine treatment and duration of hypotension. We demonstrated that the key factor in this method is duration of colchicine treatment. Our report should facilitate the establishment and studies of iPS cells of new species.
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