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Effect of SM22a on Microfilament Remodeling of VSMC after Serum Deprivation
Yun-Hui Cheng, Mei Han*, Jin-Kun Wen
Hebei Laboratory of Medical Biotechnology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Abstract: Smooth muscle 22 alpha (SM22a) is a marker of vascular smooth muscle cells (VSMC). In order to investigate the effect of SM22a on microfilament remodeling， antisense SM22a was used in present study. The change of microfilaments in VSMC was observed by immunofluorescence and transmission electron microscopy (TEM)， and the ability of migration of VSMC was measured by using a monolayer wounding model. The results demonstrated that the expression of SM22a and SM a-actin was induced quickly after serum starvation and the microfilaments were remodulated from discrete network to well arranged and dense bundles. However， after VSMC was transfected by antisense SM22a recombinant plasmid， reorganization of cytoskeleton by serum starvation was blocked， and the ability of migration of VSMC was declined. These data suggested that SM22a plays a key role during microfilament remodeling.
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