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Expression Spike Protein and Membrane Protein of SARS-coronavirus in the Insect Baculovirus
Shi-Hai Jia, Bo Zhou, Jun Wu, Mu-Wang Li, Min-Hui Pan1, Cheng Lu1, Xue-Xia Miao, Yong-Ping Huang*
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 1Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China
Abstract: SARS-Coronavirus is the pathogen of human severe acute respiratory syndrome. Studies on other species of coronaviruses indicated that the spike protein (S protein) and membrane protein (M protein) are the main structural proteins of the virus. As recombinant M protein and S protein can be used as antigens to detect coronavirus infection and to generate vaccines， the expression of these two genes is definitely necessary. In the current study， the genes of the two proteins were cloned and recombined into the genome of insect baculovirus. Then， the S protein and M protein were expressed in the insect cell lines by using the recombinant baculovirus， and the GFP fusion protein hints that M protein locates on the membrane of cells.
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