Home > Browse Issues > Vol.29 No.6
Isolation and Culture of Mouse Embryonic Stem Cells of Kunming Species in Vitro
Yu Li*, Lin Liang, Zhen-Fei Wang, Rui-Zhen Jia, Bao-Zhen Dai, Yao Li
Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Huhhot 010021, China
Abstract: To investigate the method of establishing embryonic stem (ES) cell line from Kunming species mouse， inner cell masses (ICMs) isolated by immunosurgery from 4.5 d blastocysts of Kunming species mouse were cultured on the fetal mouse fibroblast cell feeder layers. The ES cell-like colones were passaged by trypsin-EDTA digest method to 8th generation. Identified by phasecontrast microscope observation and AKP staining， the obtained ES-like cells proved to be AKP intensively positive and able to form typical ES-like colones. This research confirmed that immunosurgery is suitable for establishing ES cell lines from Kunming species mouse and the cells obtained still maintain the main characteristics of ES cell.
CN
EN