The Effect of FHL2 on the Malignant Biological Behaviors of Hunman Nasopharyngeal Carcinoma Cell Line 5-8F and Its Related Mechanism

Abstract: The transient transfection method was employed to down-regulate the FHL2 gene expression in human nasopharyngeal carcinoma 5-8F cells. qRT-PCR was used to detect the levels of FHL2， c-myc and β-catenin mRNA in each group of Transfected NP-69 and 5-8F cells. Meanwhile， the levels of FHL2， c-myc and β-catenin protein were detected by Western blot. The cell proliferation ability， cloning ability and migration ability in transfected cells were detected by CCK-8 method， cell clone formation experiment， wound healing test and Transwell assay， respectively. Western blot results showed that the level of FHL2 in nasopharyngeal carcinoma cell line 5-8F was obviously higher than that in non cancerous immortalized human nasopharyngeal epithelial cells NP- 69. After down-regulation of FHL2 gene expression， the abilities of proliferation， migration and invasion of 5-8F cells were significantly inhibited (P<0.05). The results from qRT-PCR and Western blot showed that the levels of FHL2 mRNA and protein in transfected siRNA cells were significantly lower than that of unrelated sequence group and blank control group (P<0.01). Moreover， the levels of c-myc， β-catenin mRNA and protein in the transfected 5-8F cells were significantly decreased compared with NC group and control group (P<0.01). Together， the expression of FHL2 gene， a potential oncogene was up-regulated in nasopharyngeal carcinoma 5-8F cells. FHL2 gene might regulate the germination and development of nasopharyngeal carcinoma through Wnt signaling pathway， and the abilities of cell proliferation， migration and invasion were significantly suppressed if FHL2 gene was knockdown in the 5-8F cells.

CSTR: 32200.14.cjcb.2018.05.0018