Effects of Deoxynivalenol on Proliferation and Cell Cycle Distribution of Human Gastric Carcinoma Cell Line

Abstract: To explore the putative effects of deoxynivalenol (DON) on the proliferation and the cell cycle distribution of SGC-7901 and BGC-823 cells in vitro. SGC-7901 and BGC-823 cells were treated with DON at different concentrations for 72 h. The effects of DON on the cell proliferation and cell cycle distribution of the two cell lines in vitro were determined with MTT and flow cytometric (FCM) DNA analysis， while that on the expression of P21^WAF/CIP1 and cyclin E at protein level were studied with immunocytochemical (ICH) staining and Western blotting. MTT results showed that DON treatment could significantly inhibit the proliferation of the two cell lines. The inhibition rate of DON at 100， 500 and 1 000 μg/L was 4.28%， 36.20% and 45.35% for SGC-7901 and 14.89%， 32.30% and 51.61% for BGC-823 respectively. FCM cell cycle analysis revealed that G₂/M arrest could be induced by 1 000 μg/L DON treatment for 72 h for both cell lines. Within the concentration range from 100 μg/L to 1 000 μg/L， DON could significantly increase the expression of P21^WAF/CIP1. A significant dose-effect correlation could be found between DON concentration and the intensity of P21^WAF/CIP1 expression at protein level for both cell lines (SGC-7901: r=0.886， P<0.01; BGC-823: r=0.943， P<0.01). While the expression of cyclin E was significantly decreased by DON in a dose-dependent way in the two cell lines (SGC-7901: r=-0.923， P<0.01; BGC-823: r=-0.854， P<0.01). The results of immunocytochemical staining and Western blotting confirmed that DON could increase the expression of P21^WAF/CIP1， while decrease that of cyclin E in both two cell lines. Thus， the results in this study suggested that DON could significantly inhibit the proliferation of gastric carcinoma SGC-7901 and BGC-823 cells in vitro， and the possible mechanism of the proliferation inhibiting effects of DON may be G₂/M arrest and the increase of the expression of P21^WAF/CIP1 and decrease of cyclin E. No significant differences were found in the effects of DON on the two different gastric carcinoma cell lines.

CSTR: 32200.14.cjcb.2007.05.0021