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SF-1 Induces the Proliferation of Mouse Endometrial Stromal Cells In Vitro and Its Mechanism
Mou Chunfeng, Liu Xueqing, He Junlin, Chen Xuemei, Gao Rufei, Wang Yingxiong, Ding Yubin*
Laboratory of the Reproductive Biology, School of Public Health and Management,Chongqing Medical University, Chongqing 400016, China
Abstract: Mouse endometrial stromal cell (mESC) was isolated and identified in this study. Forced expression of steroidogenic factor-1 (SF-1) in mESC induced proliferation increase and upregulation of protein expression in proliferation marker genes PH3 (phospho-histone 3) and PCNA (proliferating cell nuclear antigen) and acitivation of PI3K-AKT-mTOR signaling pathway. The PI3K signaling pathway inhibitor LY294002 and mTOR signal pathway inhibitor Rapamycin decreased the expression of pAKT S473， pmTOR S2448， pp70S6K T389， pS6 S235/236 and p4E-BP1 T37/46， which were induced by forced expression of SF-1 in primary cultured mESC. Our study provided preliminary evidences that SF-1 expression might associated with mESC cell proliferation.
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