The Effect and Mechanism of Galectin-7 on Migration and Invasion of the Esophageal Squamous Carcinoma Cell Line Eca-109

Abstract: To explore the extracellular function and mechanism of Galectin-7 in the development of esophageal Eca-109 cell line， Western blot was used to detect the expression of Galectin-7 in the concentrated cell culture supernatant， and Eca-109 cell line were incubated with human recombinant Galectin-7 in different concentrations. Western blot， Real-time quantitative PCR， scratch test and MTT were used to analyze the expressions of MMP-9， p38 and pp38 in Eca-109 cell and the impact of Galectin-7 on the invasion and the proliferation of Eca-109 cell. The result testified the existence of Galectin-7 in the cell culture supernatant， and the expressions of MMP-9 and pp38 were promoted by Galectin-7 and the ability of invading of Eca-109 was improved. Taken together， our results suggested that in Eca-109 cell line， Galectin-7 was secreted to the extracellular， and induced the expression of MMP-9 through activating the p38 MAPK pathway by binding the specificity glycosylated receptor in the cell membrane.

CSTR: 32200.14.cjcb.2014.12.0003