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Colocalization of Cdc42 and G-actin during Xenopus Oocyte Cytokinesis
Da-Ye Cheng1,2*, Bin Liang1,3*, Feng Li1**
1Key Laboratory of Cell Biology of Ministry of Public Health of China, China Medical University, Shenyang 110001, China; 2Transfusion Department, First Affiliated Hospital, China Medical University, Shenyang 110001, China; 3
Abstract: Study the colocalization of active Cdc42 and G-actin during Xenopus oocyte cytokinesis. GFP-wGBD mRNA and rhodamine-594-tubulin， alexa-488-G-actin and rhodamine-594-tubulin， GFP- wGBD mRNA and alexa-594-G-actin were microinjected into Xenopus oocytes respectively. Employ confocal microscopy and time-lapse experiments to observe localization of active Cdc42 and G-actin as well as colocalization of active Cdc42 and G-actin during oocyte cytokinesis. Results showed that active Cdc42 colocalized with G-actin spatially and temporally， and indicated active Cdc42 might tightly correlated with G-actin during Xenopus oocyte division processs.
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