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Chicken Egg Extracts Promote Increased Expression of Pluripotent Gene OCT4 and NANOG in 293T Cells
Ruan Guangping, Wang Jinxiang, Yao Xiang, Pang Rongqing, Cai Xuemin, He Jie, Zhao Jing, Pan Xinghua*
Stem Cell Engineering Laboratory, Kunming General Hospital of PLA, Kunming 650032, China
Abstract: We find an egg extract that has the role of promoting 293T cells to express pluripotent genes， and it will have broad application prospects in cell biology. Egg-white， egg-yolk and whole-egg extracts were used for the penetration induction of the 293T cells. At different times after induction， OCT4 and NANOG pluripotent gene changes were detected in cell RNA. Ten days after induction， methylation sites changes of OCT4 and NANOG gene were detected in cell DNA. The egg-white， egg-yolk and whole-egg extracts had the role of promoting the growth of 293T cells. After the three extracts permeated the 293T cells， OCT4 and NANOG gene expressions increased to varying degrees. OCT4 and NANOG genes appeared demethylation， and gene expression opened. Chicken egg extracts have the roles to increase OCT4 and NANOG gene expression in 293T cells and may be agents to induce somatic cells into pluripotent cells.
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