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Beta 2 Microglobulin Expression in Bone Mesenchymal Stem Cells
Fan Shiyang, Jin Haidong, Dai Bing, Pan Jun*
Department of Orthopedics, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China
Abstract: The study explores beta 2 microglobulin (β2M) expression in bone mesenchymal stem cells (BMSCs) by means of flow cytometry technique and laser confocal microscope. SD rat BMSCs in the same condition have been divided into A and B groups. Group A is undifferentiated BMSCs， while group B is induced in chondrogenesis medium for 1 week， and both two group using flow cytometry and laser confocal microscope to detect the expression of β2M. The results of both flow cytometry and laser confocal microscope show that group A is significantly lower than group B in β2M expression. All the results suggested that undifferentiated BMSCs posses low immunogenicity， while BMSCs that induced in chondrogenesis medium for 1 week obviously increased its immunogenicity.
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