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Variations of Epigenetic Characteristics of Germinal Ridge between Male Embryos and Female Embryos
Deng Yujin, Xiao Xiong, Shen Xinyi, Song Kun, Yang Lishan, Li Yuemin*
Chongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology,Southwest University, Chongqing 400715, China
Abstract: When mouse embryonic fibroblasts (MEFs) co-incubated with extracts of embryonic stem cells (ESCs) to reprogram into the induced pluripotent stem cells (iPSCs)， the origin of iPSCs could be rapidly and easily identified based on the different gender of MEFs and embryonic stem cells. Different sexual MEFs were isolated from different sexual Kunming mouse embryos at 13.5 dpc (days post coitum) which were identified according to the morphological differences between the male germinal ridge and the female germinal ridge. A pair of primers were designed according to the core gene sequence of special sex-determining region of Y-chromosome (SRY gene) of male mouse， and DNA samples obtained from different sexual MEFs were amplified by PCR to test and verify the differences in morphology between the different sexual germinal ridge. The results indicated that the gender of MEFs which were identified with sex identification with PCR was consistent with that of mouse embryos at 13.5 dpc. Different sexual MEFs manifested the typical characteristics of fibroblasts， and there were no difference in morphology between the male MEFs and the female MEFs. Therefore， a large number of different sexual MEFs could be isolated from different sexual mouse embryos at 13.5 dpc which were identified according to the morphological difference between the male germinal ridge and the female germinal ridge.
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