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Isolation of Zygotes and Embryos in Phalaenopsis
Wu Chenghou1,2, Li Dongmei3, Tian Huiqiao1*
1School of Life Sciences, Xiamen University, Xiamen 361005, China; 2Guangzhou Institute of Landscape Gardening, Guangzhou 510405, China; 3Shunde Polytechinic, Shunde 528300, China
Abstract: Zygotes， proembryos and maturing spherical proembryos were isolated from Phalaenopsis hybrid embryo sacs by the method of manual microdissection after a pretreatment of combinnating enzymatic maceration with shaking the ovules. The enzymatic maceration was under 0.7%~1.3% cellulase， 0.6%~1.0% pectinase， 10% mannitol， pH5.8 for about 20~30 min. Developed suspensor haustorium was discovered on the micro-dissected embryos from three-cell stage to maturing spherical.
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