Home > Browse Issues > Vol.33 No.6
Studies on Meiosis of Pollen Mother Cells in Photoperiod-temperatureSensitive Genic Male Sterile Wheat Line BS366
Jian-Qing Fan1,2, Li-Ping Zhang1,2*, Chang-Ping Zhao1,2*, Chen-Guang Xu1,2, Ling-Yun Wang1,2,Shao-Hua Yuan2
1Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing 100097, China;2School of Life Science, Capital Normal University, Beijing 100048, China
Abstract: In this paper， cytological studies were conducted on pollen mother cells meiosis of photoperiod temperature sensitive genic male sterile wheat line BS366 by using carbol fuchsin and chromosome squash technique. Some abnormalities of meiosis were observed in stertile condition. They were lagged chromosomes， chromosome bridges， scattered chromosomes and micronucleus. During meiosis II， chromosome segregations were not synchronous. At dyad stage， the cell plate was out of flatness and the cell was incomplete divided. At tetrad stage， the tetrad cells were not coincident with each other in size. The percentage of abnormal tetrad was 62.88%， and 89.5% of matured pollen grains were sterile. Abnormal chromosome behavior and cell morphology during meiosis may be the important reason for fertility reduction of photoperiod-temperature sensitive genic male sterility wheat pollen.
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