Home > Browse Issues > Vol.33 No.2
Inducement of High Frequency Mitotic Synchronization and Studies on Cell Division Cycle Proteome of Root Meristem in Wheat
Lian Wang, Feng-Ying Yin, Shuang Duan, Fu Gan, Zhen-Ying Wang*, Yong-Kang Peng*
College of Life Science / Tianjin Key Laboratory of Cyto-genetical and Molecular Regulation ,Tianjin Normal University ,Tianjin 300387, China
Abstract: In this study， Hu and APM are utilized to increase the frequency of mitotic synchronism on meristematic tissue in wheat. The frequency of induced synchronous prophase is 20%， metaphase is 79%， anaphasetelophase is 27%. 2D SDS-PAGE analysis showed that the composition of mitotic cyclin in wheat was changed periodically. In comparison with the map of interphase， 5 different protein spots (37 kDa/pI 6.6， 38 kDa/pI 6.8， 34 kDa/pI 7.2， 38 kDa/pI 7.5 and 15 kDa/pI 6.9) appeared in the map of prophase， 1 protein spot (28 kDa/pI 5.2) disappeared. In the map of metaphase， 1 protein spot (21 kDa/pI 6.3) appeared and 2 different protein spots (51 kDa/pI 7.3 and 23 kDa/pI 6.1) disappeared. After meristematic cells turned into anaphase-telophase， 4 different protein spots (37 kDa/pI 6.6， 51 kDa/pI 7.3， 23 kDa/pI 6.1 and 43 kDa/pI 6.6) appeared， and 1 protein spot (21 kDa/ pI6.3) was lost. In the whole cell cycle， 4 protein spots (37 kDa/pI 6.6， 51 kDa/pI 7.3， 23 kDa/pI 6.1 and 21 kDa/ pI 6.3) appeared and disappeared periodically. 2 protein spots were identified by MS/MS. spot 6 was identified as chromosome segregation protein SMC， and spot 9 was identified as helicase protein. These identified protein spots involved in chromosome formation and segregation， DNA duplication and energy metabolism.
CN
EN