The Effect of Ovariectomy on the Ability of Multidirectional Differentiation of MSCs from SD Rats

Abstract: To investigate the effects of ovariectomy on the ability of multidirectional differentiation into osteoblast， adipocyte and chondrocyte of Sprague-Dawley (SD) rats’ mesenchymal stem cells (MSCs) was compaired by the differentiatial condition of the MSCs derived from the osteoporosis (OP) rats and normal rats with and without induced conditions. The experiment was divided into four groups: normal group， normal induced group， OP group， and OP induced group. The ability of multidirectional differentiation was detected after treated with or without osteoblast-induced， adipocyte-induced or chondrocyte-induced conditions， respectively. The results demonstrated that the ability of osteogenic and chondral differentiation of MSCs from normal rats was stronger than that from OP rats without the induced conditions， but the ability of adipose differentiation was lower. With the induced conditions， the reaction to osteoblast-induced and chondrocyte-induced agent of MSCs from normal rats was stronger than that from OP rats， but the reaction to adipocyte-induced agent was lower. In conclusion， compared with MSCs from normal rats， the ability of osteogenic differentiation and the reaction to osteoblast-induced agent of MSCs from OP rats decreased， the ability of differentiation into adipocyte and reaction to chondrocyte-induced agent of MSCs from OP rats increased， and the reaction to chondrocyte-induced agent decreased.

CSTR: 32200.14.cjcb.2012.01.0009