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Isolation and Preliminary Analysis of the Receptor of Helicoverpa armigera NPV Fibroblast Growth Factor
Xiang Li, Ai-Hui Jin, Huan Zhang, Jian-Xin Lu*
Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou 325035, China
Abstract: Fibroblast growth factor (FGF) is a key regulator of developmental processes affecting the growth， differentiation， and migration of many cell types by activating the receptor and the signal pathway. Baculoviruses such as Helicoverpa armigera nucleopolyhedrovirus (HearNPV) also encode a FGF homolog showing chemotactic activity. Here we cloned and analyzed the partial sequence of a fgf receptor (Habtl) from Helicoverpa zea cell line， which is permission for HearNPV infection， by RT-PCR with the primers designed based on the sequences of the FGF receptor of Bombyx mori and Spodoptera frugiperda (Bmbtl and Sfbtl， respectively). Sequence analysis showed that Habtl had a high identity to Bmbtl and Sfbtl. Knock-down of Habtl expression with special siRNA showed that Habtl played an essential role for HearNPV FGF mediated chemotation and in virus infection.
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