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Influence of Enhanced Ultraviolet-B Radiation on Microtubule Cytoskeleton in Wheat Root-tip Cells
Ai-Hua Guo, Li-Mei Gao, Yong-Feng Li, Jing-Ru Zhai, Rong Han*
Institute of Cell Engineering, College of Life Science, Shanxi Normal University, Linfen 041004, China
Abstract: In order to further investigate the effects of ultraviolet-B (UV-B) radiation on the relationships between microtubule arrangements and 慞artition-bundle division? the dynamic variation of microtubules in wheat root-tip cells exposed to enhanced UV-B radiation (10.08 kJ穖?穌?) was studied with confocal laser scanning microscopy (CLSM). The results showed that the microtubule arrays presented a periodicity during mitosis of wheat root-tip cells， the obvious microtubule cycle and high fluorescence intensity were observed in control group; However， under the condition of enhanced UV-B radiation， the structure of microtubules were disorder while microtubules appeared a lot of abnormal arrangements. The cortical microtubules changed orientation to network and depolymerized significantly to stick and spot; the cells had abnormal structure of double preprophase bands; the arrangement of spindle microtubules showed accumulation and the phragmoplast microtubules were dispersivity or disappeared due to enhanced UV-B radiation.
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