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Effects of Cortex lycii Extract on the Function of Endothelial Progenitor Cells
Fei-Xia Shen*, Guang-Ming Chen1, Zhen Ye2, Sheng-Jie Ge, Lian-Song Ni
Department of Endocrinology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China; 1 Department of Endocrinology, Central Hospital of Jinhua, Jinhua 321000, China; 2 The First Affiliated Hospital, Zhejiang
Abstract: To observe the effects of Cortex lycii extract on adhesive， migratory， and proliferation activities of endothelial progenitor cells (EPCs) derived from human umbilical cord blood cells， and to investigate the protective effects of Cortex lycii extract on vascular endothelial cells from high glucose. EPCs were derived from human umbilical cord blood by Ficoll gradient centrifugation. The cells were treated with normal glucose (5.56 mmol/L glucose， NG )， high glucose (30 mmol/L glucose， HG)， and HG with different concentrations of Cortex lycii extract (1 g/L， 2 g/L and 4 g/L). EPCs were characterized as double positive fluorescent staining with UEA-I and DiLDL， as well as double positively expressing ecNOS and Flk-1. Adhesive， migratory and proliferation activities of EPCs were measured by replanting ability on fibronectin-coated dishes， CCK-8 assay and modified Boyden chamber assay. Our data shown that: (1) the adhesive， migratory and proliferative activities of EPCs decreased markedly HG group when compared to NG; (2) the adhesive， migratory and proliferative abilities of EPCs were significantly resumed in Cortex lycii extract groups， and in 2 g/L Cortex lycii extract group， the migratory and proliferative abilities of EPCs were markedly improved. In conclusion， Cortex lycii extract can partially resume the adhesive， migratory and proliferative abilities of EPCs from inhibition of high glucose. Cortex lycii extract may protect vascular endothelial cells from glucotoxicity.
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