Diazoxide Affects the Expression of GRP78 and CREB2 in Rats^，sKidneys after Hypotheric Perservation

Abstract: o investigate the protective effect of diazoxide (DE) on rat抯 kidneys suffered from hypothermic preservation and the possible mechanisms. Forty-eight SD rats were randomly divided into six groups (n=8): control group (Celsior cardioplegic solution) with preservation 0， 24， 48 h， and experiment group (20 mmol/L DE in the Celsior cardioplegia) with preservation 0， 24， 48 h. The quantity of malonaldehyde (MDA) and the activity of superoxide dismutase (SOD) of the kidneys of each group were measured after 0， 24， 48 h， and we also observed the expressions of glucose regulated protein-78 (GRP78) and cyclic AMP response element binding protein-2 (CREB2) on rat kidney after hypothermic preservation by immunohistochemistry and Western blot at that times. Compared with the control group， the quantity of MDA was significantly lower in experiment group in 24 h and 48 h respectively (P<0.01， P<0.05)， the activity of SOD was significantly higher in experiment group (48 h) (P<0.05)， and the expression of GRP78 and CREB2 were higher in experiment group in 24 h and 48 h respectively (P<0.05). This study demonstrates that DE can reduce rat renal injury via up-regulation of GRP78 and CREB2. The effects of DE could initiate the endoplasmic reticulum stress to protect the kidney.

CSTR: 32200.14.cjcb.2010.02.0012