Effects of Intraperitoneal Injection of Fuantai-03 on Metastasis-related Genes and Metastasis in Mouse Lewis Lung Carcinoma and B16 Melanoma

Abstract: Western blot analysis and immunohistochemistry protocol were performed to assay the expressions of CD44 and nm23-H1; mouse Lewis lung carcinoma (LLC)， a spontaneous tumor metastasis model， and mouse B16 melanoma (B16)， a experimental tumor metastasis model， were utilized to study the effects of intraperitoneal (ip) injection of Fuantai-03 (FAT-03) on cancer metastasis. The results showed that FAT-03 resulted in down-regulation of expression of CD44， and up-regulation of expression of nm23-H1 in vitro and in vivo， and that the growth inhibition rates of primary tumors of LLC in the FAT-03 [10.0， 20.0 mg/(kg昫)， ip， for 14 days]-administered mice were 53.8% and 61.3% [vs 58.8% of cyclophosphamide (CY) ]， and the liver metastasis inhibition rates (MIR) of LLC were 37.4% and 76.5% (vs. 64.5% of CY)， and the lung MIR of B16 were 51.4% and 63.7% (vs 44.0% of CY， and 88.1% of 10.0 mg/kg FAT-03+CY)， respectively. These observations qualify FAT-03 as an interesting lead component to prevent cancer metastasis; FAT-03-induced change of expressions of metastasis-related genes may be partially responsble for its inhibitory effect on metastasis.

CSTR: 32200.14.cjcb.2010.02.0011