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Effects of Lipoxin A4 on Proliferation and Apoptosis of K562 and HL-60 Leukemic Cells
Xiao-Yan Zhou1,2, Ping Wu2, Zhen-Yu Cai1, Fang-Yun Xu1, Hong-Mei Wang1, Du-Yun Ye2*
1Department of Pathophysiology, Medical College of Nanchang University, Nanchang 330006, China; 2Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract: Inflammation is a critical component of tumour progression. Lipoxins (LXs) are approved for potent anti-inflammatory properties. However， there are few reports dealing with their effects on cancer. In this study， we tested their effects on HL-60 and K562 leukemia cells. Western blot and real time PCR were used to examine the expression of lipoxin A4 receptor (ALX). Cellular proliferation and cycle were determined via cell counting kit-8 assay (CCK-8) and propidium iodide staining. Apoptosis was assessed by the annexin V assay. We discovered LXs inhibited the proliferation of HL-60 and K562 cells， increased the number of cells in G0/G1 phase， decreased that in S phase， and induced the apoptosis of HL-60 and K562 cells. It hinted LXs inhibited the proliferation of HL-60 and K562 cells and the mechanism might be associated with cell cycle arrest in G0/G1 phase and induction of apoptosis.
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