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Effects of Cryoprotectants and Durations of Cryopreservation on Post-thaw Cytoactive of Insect Cell Line NIAS-MaBr-85
Yan Ma, Wei-Feng Ding, Ying Feng*, Xin Zhang, Tao Ma
Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China
Abstract: In order to evaluate the effects of different cryoprotectants and durations on post-thaw cytoactive of insect cell line NIAS-MaBr-85， 3 kinds of cryoprotectants (Formula I: 10% DMSO + 90% FBS; Formula II: 10% DMSO; Formula III: 10% glycerol) were used to preserve cells in liquid nitrogen. The post-thaw viability and circularity of cells were examined at 1， 3， 9， 17， 20， 28， and 38 months separately. The results showed that the post-thaw viability of cells decreased with the time of cryopreservation expended. In short-term cryopreservation， the effects of different cryoprotectants on post-thaw cytoactive of NIAS-MaBr-85 were not significant. In long-term cryopreservation， the effect of preservation of 10% DMSO was better than that of 10% glycerol. The effect of supplement of 90% FBS on preserving the post-thaw biological characteristics of cells was not obvious. Comparing the influence of the length of cryopreserved time， different cryoprotectants had a statistically significant influence on the post-thaw biological characteristics of NIAS-MaBr-85 cells.
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