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Effect of DAPI Stain on Elastic Properties of A549 Cells
Zhi-Yi Ye*, Li Zhang, Xia Fan
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education,Bioengineering College,Chongqing University, Chongqing 400044, China
Abstract: Mechanical properties of living cell can be determined by using atomic force microscopy (AFM). In this study， the A549 cells line and A549 cell line which was dyed by DAPI were measured by AFM. Here we report the effect of DAPI on the A549 cell mechanical properties. Cells were incubated with DAPI， and individual cell stiffness was tracked with AFM. We used AFM to measure quantitatively the local mechanical properties of A549 cells. To get quantitative information on sample elasticity， forces vs. indentation curves were got. The curves were then fitted with the Hertz model， yielding Young's modulus values of 3.21±1.73 kPa and 3.66±2.08 kPa for the A549 cell and similar cell dyed by DAPI， respectively. There was no significant difference between the A549 cell and the similar cell dyed by DAPI stain. These observations suggest that DAPI will not lead to the change of cell's stiffness.
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