Influence of PDGF-BB and PKGIa on Morphology and Cytoskeleton of Vascular Smooth Muscle Cell

Abstract: Primary rat aortic smooth muscle cells (RASMCs) were prepared by explant-attachment method. Both the primary cells and rat pulmonary artery smooth muscle cell line CS-54 were infected with recombinant adenovirus encoding gene cGMP-dependent protein kinases I a (pAd-PKGIa) and were treated with platelet-derived growth factor-BB (PDGF-BB) to study the effects on morphology and cytoskeleton organization. Cell morphology was observed by reverse microscope and stress fiber structure was analyzed by fluorescence staining. It was found that in both CS-54 cells and RASMCs， PDGF-BB not only caused obvious change of the morphology but also disrupted the cytoskeleton organization， which was indicated by the change of the amount and the distribution of stress fibers. Infection with pAd-PKGIa and treatment of 8-APT-cGMP resumed the structure of stress fibers. These results demonstrated that PDGF-BB has an influence on morphology and cytoskeleton organization of CS-54 cells and RASMCs， and increase of the expression and the activity of PKGIa can reverse the effect.

CSTR: 32200.14.cjcb.2010.01.0015