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The Meiotic Progress of Porcine Oocytes Matured in Vitro
Ya-Qiong Huang1,2, De-Shun Shi1*, Xiao-Xi Zhang1, Shun Zhang1, Xu-Jian Chen2, Jia-Zhou Li2,Shi-Yuan Zeng2, Gui-Wen Ruan2, Shi-Hua Zhao2, Shi-Jian Tan
1Institute of Animal Reproduction, Guangxi University, Nanning 530005, China;2Yulin Normal University, Yulin 537000, China
Abstract: The meiotic progress and developmental competence of porcine oocytes matured in vitro were investigated in the present study. Most of oocytes from follicles over 2 mm in diameter were taken place germinal vesicle breakdown (GVBD) at 16 h after in vitro maturation (IVM)， in which the proportion of oocytes in the GV stage was decreased from 68.00% to 15.38%. At 20 h after IVM， the percentage of oocytes in the diakinesis stage was increased to 27.06%， and most of oocytes (69.66%) matured to the MI stage at 28 h after IVM. At 32?4 h after IVM， the proportion of oocytes in the Ana-I/Tel-I stage was up to the peak value (53.33%). The percentage of porcine oocytes in the MII stage was up to the peak value (63.64%) at 48 h after IVM. The maturation rates of oocytes (extruded the first polar body， PB1) from follicles in diameter of <2 mm， 2? mm and >6 mm were 5.12%， 66.62% and 46.71%， respectively. The cleavage rates of oocytes from follicles in diameter of <2 mm (2.86%) and >6 mm (58.69%) were significantly lower than that of oocytes from follicles in diameter of 2? mm (72.11%， P< 0.05) after IVM and parthenogenetic activation. However， the blastocyst yield of oocytes from follicles in diameter of 2? mm (28.62%) was not different from that of oocytes from follicles in diameter over 6 mm (23.46%， P>0.05). These results will provide necessary data for investigating the meiotic process and regulation of meiosis in porcine oocytes.
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