Effects of Centrifugation of Mouse Oocytes on the Localization of Meiotic Spindle and the Early Developmental Potential

Abstract: In order to evaluate the effects of centrifugation on the location of the meiotic spindle and early developmental potential of mouse oocytes， the metaphase-II mouse oocytes were centrifuged at 1 000， 3 000 and 5 000 r/min for 10 minutes. The location of the meiotic spindles of centrifuged oocytes was detected by confocal microscopy， and their early developmental potential was analyzed after parthenogenetic activation and in vitro fertilization (IVF). The results displayed that the meiotic spindles of the centrifuged oocytes showed the normal configuration， and located at the edge of cortex， adjoining the PB1. The spindle dynamics and the meiotic progress of parthenogenetic activated oocytes treated with centrifugation were consistent with control， and centrifuged oocytes kept the normal early developmental potential after parthenogenetic activation or IVF. The present results imply that the centrifugation-pretreatment of mouse oocytes before enucleation operation may improve the efficiency of enucleation without Hoffmann objective during nuclear transfer.

CSTR: 32200.14.cjcb.2010.01.0012