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Effects of 5-Aza-CdR on Cell Growth and Dnmt3a Expression in JEG-3 Human Choriocarcinoma Cell
Yuan-Hu Wang, Yu-Bin Ding, Xue-Mei Chen, Xue-Qin Liu, Ying-Xiong Wang, Jun-Lin He*
College of Public Health, Chongqing Medical University, Chongqing 400016, China
Abstract: The choriocarcinoma JEG-3 cells were treated with different concentrations (1， 4， 8， 16， 32 mmol/L) of the DNA methyltransferase inhibitor 5-Aza-CdR. Changes of cell morphology were observed under the microscope and cell proliferation was detected by MTT. The apoptosis and invasion of JEG-3 cells were specially measured by flow cytometry and Transwell chamber respectively. The expression of Dnmt3a mRNA and protein were measured. The results showed that 5-Aza-CdR induced the cell shrinkage， chromatin integrated， and inhibited JEG-3 cell growth in a dose-dependent manner. 5-Aza-CdR treatment led to cell apoptosis and the depression of cell invasiveness. Expression of Dnmt3a mRNA levels and Dnmt3a protein expression levels decreased remarkably after 5-Aza-CdR treatment.
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