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Stem Cell Factor Induces Directional Migration of Neural Stem Cells
Chun-Lei Zhou, You-Hua Wei, Jing Liu, Yan-Wen Zheng, Jun-Ke Zhang, Dan Wang,Dan Wu, Jun Su, Huan-Xiang Zhang*
Jiangsu Key Laboratory of Stem Cell Research, Department of Cell Biology,Medical College of Soochow University, Suzhou 215123, China
Abstract: Neural stem cells (NSCs) can directly migrate towards glioma. However， the mechanisms and factors that regulate migration are not well understood. In this study， we investigated stem cell factor (SCF) induced C17.2 cells migration by Boyden chamber and Dunn chamber. Compared with the control， the results of Boyden chamber showed that the number of the cells migrated to the lower well through a filter of 8-mm pore size was significantly higher than control when the SCF was added into the lower well. The Dunn chamber results indicated both the migration speed and the forward migration index (FMI) of cells exposed to SCF were significantly greater than those of cells exposed to a uniform concentration of SCF. The cell migration pattern also displayed a directed migration behavior towards the source of SCF in concentration gradients of SCF， while cells migrated randomly under conditions of uniform SCF distribution. These results illustrate that SCF induces the directional migration of C17.2 cells via the increase in the migration efficiency and the migration speed of these cells， which would provide data to understand the mechanisms of glioma-induced migration of NSCs.
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