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A Simple Method for DNA Extraction from Human Blood Clot
Li Chen*, Li Jiang, Yang Luo, Xue Zhang
The Research Center for Medical Genomics and Key Laboratory of Cell Biology Affiliated to Ministry of Public Health of China, China Medical University, Shenyang 110001, China
Abstract: The method described here enables to extract DNA from human blood clots. The key procedures included: blood clots were homogenized with 80 g/L NaCl and 10% EDTA， the homogenized samples were lysed with 500 ml of digestion buffer (10 mmol/L Tris， 1 mmol/L EDTA， 20 g/L SDS， 300 mg/ml proteinase K) and incubated for 2 h at 56 ℃， then the homogenized samples were incubated overnight at 37 ℃. This method was reliable for obtaining high quantities of DNA suited for PCR and restriction digest.
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