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OMgp LRR181-228: The Predominant Domain of OMgp in Neurite Outgrowth Inhibition
Yong-Jun Fan1, Jian Xu1, Long Li1,2, Chao-Zheng Zhang 2, Wang-Jun Wan1, Lian Yu1,2*
1College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China; 2College of Animal Science, Zhejiang University, Hangzhou 310029, China
Abstract: Several observations suggest that the leucine-rich repeats (LRR) domain of oligodendrocyte-myelin glycoprotein (OMgp) may play an important role in inhibition of neurite outgrowth and axonal regeneration after brain injury. To better understand structure-function relationships for the OMgp LRR domain and its effects in neurite regeneration， distinct OMgp leucine-rich repeat were deleted by PCR based site-directed mutagenesis. The gene deleted OMgp LRR fragments were expressed with GST protein and the expressed proteins were used to culture with NgR-expressing CHO and hippocampal neurons. Results show that the deletion of OMgp amino acid residues 25-56， 57-133，134-180 did not interrupt its effects in binding NgR-expressing CHO and inhibiting hippocampal neurons growth. But the fragment deleted amino acid residues 181-228 can only bind with NgR-expressing CHO and lost the effect of neurite outgrowth inhibition. Results suggest that the OMgp amino acid 181-228 is the predominant domain in neurite outgrowth inhibition of OMgp. The OMgp LRR fragment without amino acid residues 181-228 may be a potent reagent for encouraging regeneration in the adult central nervous system following injury.
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