The Differentiation of U937 Cells Induced by Normal Human Bone Marrow Fibroblastoid Stromal Cell Line

Abstract: To investigated the effects of normal human bone marrow fibroblastoid stromal cell line (HFCL) on the differentiation of acute myeloid leukemia cell line U937， the coculture system of leukemia cell line U937 and HFCL cells was established at first. Growth curves were detected by cell counting. Cell differentiation was determined by morphologic observation， ability of NBT cells and flow cytometric detection of the expression of CD11b， CD14， CD13 and CD33. Flow cytometry and Western blot were performed to test the changes of cell cycle and expression of P38， respectively. Compared with U937 cells without HFCL cells， the proliferation of U937 cells in direct contact with HFCL cells and with HFCL cells separated by transwell was inhibited， and NBT positive cells increased to 46.3%. The percentage of G1 phase cells of U937 cells with HFCL cells was higher than that without HFCL cells， and the percentage of S phase cells was lower. Meanwhile the expression of CD11b and CD14 increased， however， the expression of CD13 and CD33 didn't change. The expression of P38 in U937 cells with HFCL cells was higher than that in U937 cells without HFCL cells. Meanwhile， HFCL cells could enhance the differentiation of U937 cells induced by TPA. In a word， the normal bone marrow fibroblastoid stromal cells HFCL could induce the differentiation of part of U937 cells into monocyte with the increasing expression of P38 and also enhance the differentiation of U937 cells induced by TPA.
    

CSTR: 32200.14.cjcb.2004.05.0021