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Animal Models of Allergic Diseases Induced by Platanus acerifolia Pollen and Measurements of Specific Antibodies
Xiao-Dong Chen, Zhen Chen*, Shang-Quan Zhang1, Zhi-Li Wu1, Yi-Hong Wang1, Yi-Ping Li1
Respiratory Department of Huashan Hospital, Fudan Univirsity, Shanghai 200040, China; 1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Abstract: Allergic diseases of rats were induced by allergen extracted from Platanus acerifolia pollen. Firstly， rats were injected intraperitoneally and subcutaneously with the mixture of allergen and adjuvant. Subsequently， these rats were challenged with aerosolized allergen. After the last inspiration， rats were killed and their serums were separated. Specific IgE and IgG in serums were measured by ELISA or Western blotting. The results revealed the existence of specific IgE and IgG.
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