Improvement of Plant Regeneration from Mustard (Brassica juncea var. tumida) Cultured in vitro

Abstract: An efficient regeneration system of mustard (Brassica juncea Czern. et Coss. var. tumida Tsen et Lee cv. Zhetong No. 1) was developed in this study. The results showed that regeneration ratio was fifty percent， when supplemented with 2 mg/L 6-BA and 0.2 mg/L 2，4-D on Murashige and Skoog (MS) medium and hypocotyl as explant; while the regeneration ratio was sixty seven percent， when supplemented with 1.5 mg/L CPPU and 0.2 mg/L 2，4-D on MS medium， and the optimal explant was cotyledon with petiole. Moreover， the result indicated that CPPU were more effective in callus induction and shoot regeneration. Interestingly， the elongation of regenerated buds was inhibited when supplemented with 0.25-1 mg/L GA₃， and the phenomenon of polarity was observed in regeneration of cotyledon petiole and hypocotyl explants. This may help in the gene transformation to improve agronomic characters in mustard for breeding programmes.

CSTR: 32200.14.cjcb.2004.04.0026