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The Effects of EGF， bFGF and PHGF on the Growth of Rat Skeletal Muscle Satellite Cell
Su-Xia Shao1, Hong-Jun Ma1, Chun-Fang Zhao1, Lei Zhang1*, Li-Fen Zheng2, Yun-Li Yan2, Ai-Yuan Hu1
1Department of Histology and Embryology, 2Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Abstract: MTT method， flow cytometry， BrdU incorporation and PCNA immunocytochemistry were used to study the effects of basic fibroblast growth factor (bFGF)， epidermal growth factor (EGF) and hepatocyte growth-promoting factor (PHGF) on the growth of rat skeletal muscle satellite cells. The results showed that bFGF and PHGF enhanced the proliferation of satellite cells. There was no difference between bFGF and PHGF. The best concentration of bFGF and PHGF was 5 mg/L and 10 mg /ml， respectively. Compared with other growth factors， PHGF was cheaper and easy to obtain， so it was suited to apply. The effect of EGF appeared not to be significant.
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