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The Effects of Extremely Low-frequency Pulsed Electrical Field on NGF Receptors and Outgrowth in PC12 Cells
Xiao-Jun Yu, Jun-Ying Wang, Hong-Feng Zhang*, Shu-De Chen1
School of Life Science, East China Normal University; 1Key Laboratory of Ministry of Education for Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China
Abstract: PC12 cells were exposed to low-frequency pulsed electrical field (f=50 Hz， t=20 ms， Epp=1 V/m) to study the possible effects of electrical field exposure on nerve growth factor receptor (NGFR) and outgrowth in PC12 cells. The results showed that pulsed electrical field could obviously induce the clustering of NGFR. The clustering effect was strongest after exposure to low-frequency pulsed electrical field for 15 min， just as the effect of NGF. But it was weakest after exposure for 5 min. It suggested that receptor in the cytoplasmic membrane would be the site where low-frequency pulsed electrical field interacted with cells. By an image-analysis software， it was showed that low-frequency pulsed electrical field promoted outgrowth of PC12 cells.
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