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Culture and Related Characteristics of Macaque Ear Fibroblasts Used for Cloning
Cai-Xia Yang1, Yu-Qi Wu1, Zhi-Ming Han1, Zi-Yu Zhu1,2, Da-Yuan Chen1*
1State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; 2College of Life Science, Soochow University, Suzhou 215006, China
Abstract: Fibroblasts derived from macaque (Macaca mulatta tcheliensis) ear were successfully cultured with a normal tissue culture method. The cultured cells were analyzed by their morphology， skeleton， chromosomes and cell cycle. Results demonstrated that these cultured cells were typical fibroblasts with normal morphology， cell skeleton and karotype， and G0/G1 rate increased with the confluence of cells. Marked difference existed in the G0/G1 and S stage rate of 70%-80% and 90%-100% confluence. The culture and characterization analysis of macaque fibroblasts provided not only a ready supply of somatic cells for the intraspecies and interspecies cloning of macaque but also fundamental conditions for somatic transgene and other related fields.
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