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Influence of Hirudin on the Activation of Matrix Metalloproteinase-2 in Human Umbilical Vein Endothelial Cell
Min Wang*, Wei-Dong Zhang1, Lian-Qun Cui, Xiao-Jun Wang, Yu-Qin Yan2, Feng-Ju Qin2
Department of Cardiology, Shandong Provincial Hospital, the Clinical Medical College of Shandong University, Jinan 250021, China; 1Department of Basic Research , Shandong Medical Science Institute, Jinan 250031, China; 2Department of Biomedicin
Abstract: To investigate whether hirudin has effects on the production and activation of matrix metalloproteinase-2 (MMP-2) in cultured human umbilical vein endothelial cell (HUVEC) induced by thrombin. HUVECs were isolated from primary human umbilical vein. The cultured 2-5 passages were incubated with thrombin (4.0 ku/L)， while hirudin (6.0 ku/L) and heparin (6.0 ku/L) were added respectively except for control group. At different time， the expression of MMP-2 was determined by RT-PCR and immunocytochemistry. It was found that thrombin could upregulate the expression of matrix MMP-2 on HUVEC with time-dependent pattern (1-48 h). Hirudin showed remarkably inhibition to activation of MMP-2.
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