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The Distribution of CD105+ Cells with Characteristics of Mesenchymal Stem Cells in Diverse Fetal Tissues and Organs
Hong Guo, Jie-Wen Liu, Shao-Guang Yang, De-Jun Cao, Jin-Hua Liu, Lian-Ming Liao, Chun-Hua Zhao*
State Key Laboratory of Experimental Haematology, Institute of Haematology and Blood Diseases Hospital, and Tissue Engineering Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
Abstract: To investigate whether there are CD105+ mesenchymal stem cells (MSCs) in fetal diverse tissues and organs besides MSCs derived from bone marrow， we assessed the distribution of CD105 in fetal diverse tissues and organs， and isolated CD105+ cells from various tissues. CD105+ cells were induced to differentiate into adipocytes and osteoblasts. The results showed that CD105+ cells distributed in vascular and mesenchymal cells of heart， liver， spleen， lung， kidney， thymus gland， pancreas， blood vessel， skeletal muscles and derma. The sorted CD105+ cells could differentiate into adipocytes and osteoblasts. During differentiation of MSCs into adipocytes， CD105 expression decreased， strongly supporting that CD105 was a marker of MSCs. Dexamethasone could enhance adipogenesis and the production of triglycerides in the medium of differentiation into adipocytes. During osteogenesis， some needle-shaped crystal calcium deposition similar to bone spicules was also observed both inside and outside of the cytoplasm. These results demonstrated that CD105+ MSCs distributed in various fetal tissues. The increase of content of triglycerides in the medium of differentiation into adipocytes and calcium deposition inside the cytoplasm indicated CD105+ MSCs reside in diverse tissues may be related with some pathologic progress.
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