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Construction of HCA520 and GFP Fusion Gene Eukaryotic Expression Recombinant and Analysis Its Cell Location
YANG Mei Xiang1,2, QU Xun1*, HAN Ke Jun2, MA Dao Xin1, CHEN Wei Feng2
1Basic Research Institute of Clinic Medicine, Qilu Hospital, Shandong University, Jinan 250012; 2 Department of Immunology, Peking University Health Science Center, Beijing 100083, China
Abstract: Using PCR technique to obtain HCA520 encoding gene and cloned into the pGEM-T Easy vector. The fusion gene was identified by DNA sequencing. The identified gene was subcloned into the pEGFP-N1 vector and further identified by SalI and XhoI restriction enzymes. The confirmed recombination was transfected into the CHO cells， analysis the cell location using laser scanning confocal microscope. Most of the HCA520 protein was located in the cytoplasm. The eukaryotic expression recombination of HCA520 and its cell location analysis were valuable for further study of HCA520.
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