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Effects of Escherichia coli K88 Adhesion on Membrane Characteristics of Caco-2 Cells in vitro
GUO Tong*, XU Zi Rong
Key Lab for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute of Zhejiang University, Hangzhou 310029, China
Abstract: Caco-2 intestinal epithelial cells were used in this experiment and adhered with Escherichia coli K88. The change in the viability of the cells and the activity of membranous PLA2， the calcium concentration of cells and membrane fluidity were studied. Results showed: at 3h after the adhesion of Escherichia coli K88， the viability of the Caco-2 cells decreased significantly; the activity of membranous PLA2 and the calcium concentration of cells increased significantly; cell membrane fluidity decreased. Therefore， structure and function of Caco-2 cell membrane suffered impairment.
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