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The Different Percentage of Neural Stem Cells in Neurospheres
WANG Xiao Jing1, ZHANG Heng2*
1Institute of Cell Biology, Shandong University Medical School, Jinan 250012; 2Institute of Physiology, Shandong University Medical School, Jinan 250012, Ch
Abstract: To study the different percentage of neural stem cells quantitatively in clonal structures， "neurospheres"， which are generated by neural stem cells (NSCs) in vitro. The percentage of nestin-positive cells in neurospheres of different passages was observed by the technology of serum free and cell clone culturing and immunocytochemistry. We found that the more the passages， the less is the percentage of nestin-positive cells in neurospheres. It suggests that the neurosphers generated in vitro system are heterogeneous， and the percentage of neural stem cells in neurospheres varies significantly in different passages.
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